DCK Mouse Monoclonal Antibody [Clone ID: OTI3F5]

CAT#: CF502688

Carrier-free (BSA/glycerol-free) DCK mouse monoclonal antibody, clone OTI3F5 (formerly 3F5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DCK (RC210767, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DCK. Positive lysates LY400272 (100ug) and LC400272 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DCK monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

• 

  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-DCK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502688)

• 

  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-DCK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502688)

• 

  Anti-DCK mouse monoclonal antibody (TA502688) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DCK (RC210767).

• 

  HEK293T cells transfected with either RC210767 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DCK antibody (TA502688), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-DCK antibody (TA502688), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-DCK antibody (TA502688), (Red), compared to a nonspecific negative control antibody, (Blue).

Show all 8 images

Specifications

Product Data

• Clone Name: OTI3F5
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DCK (NP_000779) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 30.3 kDa
• Gene Name: Homo sapiens deoxycytidine kinase (DCK), mRNA.
• Database Link:
  NP_000779
  Entrez Gene 13178 MouseEntrez Gene 79127 RatEntrez Gene 1633 Human
  UniProt ID P27707
• Background: Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity. [provided by RefSeq, Jul 2008]
• Synonyms: MGC117410; MGC138632

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Purine metabolism, Pyrimidine metabolism