CCNB1IP1 Mouse Monoclonal Antibody [Clone ID: OTI4H3]

CAT#: CF502700

Carrier-free (BSA/glycerol-free) CCNB1IP1 mouse monoclonal antibody, clone OTI4H3 (formerly 4H3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCNB1IP1 (RC213849, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCNB1IP1. Positive lysates LY405381 (100ug) and LC405381 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CCNB1IP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502700)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-CCNB1IP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502700)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CCNB1IP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502700)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CCNB1IP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502700)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CCNB1IP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502700)

• Flow cytometric Analysis of Hela cells, using anti-CCNB1IP1 antibody (TA502700), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-CCNB1IP1 antibody (TA502700), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI4H3
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CCNB1IP1 (NP_878269) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 31.4 kDa
• Gene Name: Homo sapiens cyclin B1 interacting protein 1 (CCNB1IP1), transcript variant 2, mRNA.
• Database Link:
  NP_878269
  Entrez Gene 239083 MouseEntrez Gene 57820 Human
  UniProt ID Q9NPC3
• Background: HEI10 is a member of the E3 ubiquitin ligase family and functions in progression of the cell cycle through G(2)/M. [supplied by OMIM, Apr 2004]
• Synonyms: C14orf18; HEI10