RACK1 Mouse Monoclonal Antibody [Clone ID: OTI2D8]

CAT#: CF502717

Carrier-free (BSA/glycerol-free) GNB2L1 mouse monoclonal antibody, clone OTI2D8 (formerly 2D8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GNB2L1 (RC205092, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GNB2L1. Positive lysates LY401838 (100ug) and LC401838 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GNB2L1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502717)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-GNB2L1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502717)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-GNB2L1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502717)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-GNB2L1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502717)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-GNB2L1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502717)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-GNB2L1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502717)

• HEK293T cells transfected with either RC205092 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GNB2L1 antibody (TA502717), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-GNB2L1 antibody (TA502717), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-GNB2L1 antibody (TA502717), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI2D8
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GNB2L1 (NP_006089) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 34.9 kDa
• Gene Name: Homo sapiens receptor for activated C kinase 1 (RACK1), mRNA.
• Database Link:
  NP_006089
  Entrez Gene 10399 Human
  UniProt ID P63244
• Synonyms: Gnb2-rs1; GNB2L1; H12.3; HLC-7; PIG21

Reference Data

• Protein Families: Druggable Genome