ECRG4 Mouse Monoclonal Antibody [Clone ID: OTI1A10]

CAT#: CF502718

Carrier-free (BSA/glycerol-free) C2orf40 mouse monoclonal antibody, clone OTI1A10 (formerly 1A10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C2orf40 (RC206239, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C2orf40. Positive lysates LY403168 (100ug) and LC403168 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-C2orf40 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).

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  HEK293T cells transfected with either RC206239 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-C2orf40 antibody (TA502718), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Hela cells, using anti-C2orf40 antibody (TA502718), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

Specifications

Product Data

• Clone Name: OTI1A10
• Applications: FC, WB
• Recommended Dilution: WB 1:2000, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human C2orf40 (NP_115787) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 17 kDa
• Gene Name: Homo sapiens ECRG4 augurin precursor (ECRG4), mRNA.
• Database Link:
  NP_115787
  Entrez Gene 84417 Human
  UniProt ID Q9H1Z8
• Synonyms: ECRG4

Reference Data

• Protein Families: Secreted Protein, Transmembrane