PDLIM2 Mouse Monoclonal Antibody [Clone ID: OTI11A12]

CAT#: CF502731

Carrier-free (BSA/glycerol-free) PDLIM2 mouse monoclonal antibody, clone OTI11A12 (formerly 11A12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDLIM2 (RC210022, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDLIM2. Positive lysates LY411957 (100ug) and LC411957 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PDLIM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502731)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PDLIM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502731)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PDLIM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502731)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PDLIM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502731)

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  Anti-PDLIM2 mouse monoclonal antibody (TA502731) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDLIM2 (RC210022).

• HEK293T cells transfected with either RC210022 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PDLIM2 antibody (TA502731), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-PDLIM2 antibody (TA502731), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-PDLIM2 antibody (TA502731), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI11A12
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:100, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PDLIM2 (NP_067643) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 37.3 kDa
• Gene Name: Homo sapiens PDZ and LIM domain 2 (PDLIM2), transcript variant 2, mRNA.
• Database Link:
  NP_067643
  Entrez Gene 64236 Human
  UniProt ID Q96JY6
• Background: This gene encodes a member of the ALP subfamily of PDZ-LIM domain proteins. The encoded protein suppresses anchorage-dependent growth and promotes cell migration and adhesion through interactions with the actin cytoskeleton via the PDZ domain. The encoded protein is also a putative tumor suppressor protein, and decreased expression of this gene is associated with several malignancies including breast cancer and adult T-cell leukemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct
• Synonyms: MYSTIQUE; SLIM