DNAJB2 Mouse Monoclonal Antibody [Clone ID: OTI4D12]

CAT#: CF502760

Carrier-free (BSA/glycerol-free) DNAJB2 mouse monoclonal antibody, clone OTI4D12 (formerly 4D12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DNAJB2 (RC206769, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DNAJB2. Positive lysates LY422070 (100ug) and LC422070 (20ug) can be purchased separately from OriGene.

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  Anti-DNAJB2 mouse monoclonal antibody (TA502760) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DNAJB2 (RC206769).

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  HEK293T cells transfected with either RC206769 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DNAJB2 antibody (TA502760), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Jurkat cells, using anti-DNAJB2 antibody (TA502760), (Red), compared to a nonspecific negative control antibody, (Blue).

Specifications

Product Data

• Clone Name: OTI4D12
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DNAJB2 (NP_001034639) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 30.4 kDa
• Gene Name: Homo sapiens DnaJ heat shock protein family (Hsp40) member B2 (DNAJB2), transcript variant 1, mRNA.
• Database Link:
  NP_001034639
  Entrez Gene 3300 Human
  UniProt ID P25686
• Background: The protein encoded by this gene is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This encoded protein, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. [provided by RefSeq]
• Synonyms: CMT2T; DSMA5; HSJ-1; HSJ1; HSPF3