ALDH1A3 Mouse Monoclonal Antibody [Clone ID: OTI4E8]

CAT#: CF502841

Carrier-free (BSA/glycerol-free) ALDH1A3 mouse monoclonal antibody, clone OTI4E8 (formerly 4E8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALDH1A3 (RC209656, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALDH1A3 (1:500).

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ALDH1A3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Negative control E. coli lysate (Left lane) or E. coli lysate containing recombinant protein fragment for human ALDH1A3 (NP_000648) gene (the fusion of amino acids 1-100 and 413-512) (Right lane). Equivalent amounts (5 ug per lane) were separated by SDS-PAGE and then immunoblotted with anti-ALDH1A3.

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ALDH1A3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA502841)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ALDH1A3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA502841)

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  Flow cytometric Analysis of Jurkat cells, using anti-ALDH1A3 antibody (TA502841), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI4E8
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:200~2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-100 and 413-512 of human ALDH1A3 (NP_000648) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 55.9 kDa
• Gene Name: Homo sapiens aldehyde dehydrogenase 1 family member A3 (ALDH1A3), transcript variant 1, mRNA.
• Database Link:
  NP_000684
  Entrez Gene 220 Human
  UniProt ID P47895
• Background: Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. The enzyme encoded by this gene uses retinal as a substrate, either in a free or cellular retinol-binding protein form. [provided by RefSeq, Jul 2008]
• Synonyms: ALDH1A6; ALDH6; MCOP8; RALDH3

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Drug metabolism - cytochrome P450, Glycolysis / Gluconeogenesis, Histidine metabolism, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Phenylalanine metabolism, Tyrosine metabolism