PNMT Mouse Monoclonal Antibody [Clone ID: OTI1B11]

CAT#: CF502854

Carrier-free (BSA/glycerol-free) PNMT mouse monoclonal antibody, clone OTI1B11 (formerly 1B11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PNMT (RC206586, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PNMT. Positive lysates LY400946 (100ug) and LC400946 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PNMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502854)

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  Anti-PNMT mouse monoclonal antibody (TA502854) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PNMT (RC206586 ).

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  Immunofluorescent staining of HeLa cells using anti-PNMT mouse monoclonal antibody (TA502854).

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  HEK293T cells transfected with either RC206586 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PNMT antibody (TA502854), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Hela cells, using anti-PNMT antibody (TA502854), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-PNMT antibody (TA502854), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI1B11
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PNMT (NP_002677) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 30.7 kDa
• Gene Name: Homo sapiens phenylethanolamine N-methyltransferase (PNMT), transcript variant 1, mRNA.
• Database Link:
  NP_002677
  Entrez Gene 18948 MouseEntrez Gene 5409 Human
  UniProt ID P11086
• Background: The product of this gene catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. [provided by RefSeq]
• Synonyms: PENT; PNMTase

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Tyrosine metabolism