EIF4E Mouse Monoclonal Antibody [Clone ID: OTI6C3]

CAT#: CF502896

Carrier-free (BSA/glycerol-free) EIF4E mouse monoclonal antibody, clone OTI6C3 (formerly 6C3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF4E (RC207333, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF4E. Positive lysates LY400723 (100ug) and LC400723 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EIF4E monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-EIF4E mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502896)

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  Flow cytometric Analysis of fixed permeabilized cells, using anti-EIF4E antibody (TA502896), (Red), compared to an IgG isotype control, (green), and negative control (PBS), (Blue) (1:100).

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  Flow cytometric Analysis of permeabilized Jurkat cells, using anti-EIF4E antibody (TA502896), (Red), compared to an IgG isotype control, (green) (1:100).

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Specifications

Product Data

• Clone Name: OTI6C3
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human EIF4E (NP_001959) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 24.9 kDa
• Gene Name: Homo sapiens eukaryotic translation initiation factor 4E (EIF4E), transcript variant 1, mRNA.
• Database Link:
  NP_001959
  Entrez Gene 13684 MouseEntrez Gene 117045 RatEntrez Gene 1977 Human
  UniProt ID P06730
• Background: All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G (MIM 600495) (Rychlik et al., 1987 [PubMed 3469651]). [supplied by OMIM]
• Synonyms: AUTS19; CBP; eIF-4E; EIF4E1; EIF4EL1; EIF4F

Reference Data

• Protein Pathways: Insulin signaling pathway, mTOR signaling pathway