NEU2 Mouse Monoclonal Antibody [Clone ID: OTI4F4]

CAT#: CF502942

Carrier-free (BSA/glycerol-free) NEU2 mouse monoclonal antibody, clone OTI4F4 (formerly 4F4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEU2 (RC219858, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEU2. Positive lysates LY401652 (100ug) and LC401652 (20ug) can be purchased separately from OriGene.

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  Flow cytometric Analysis of Hela cells, using anti-NEU2 antibody (TA502942), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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  Anti-NEU2 mouse monoclonal antibody (TA502942) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NEU2 (RC219858 ).

Specifications

Product Data

• Clone Name: OTI4F4
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NEU2 (NP_005374) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 42.1 kDa
• Gene Name: Homo sapiens neuraminidase 2 (NEU2), mRNA.
• Database Link:
  NP_005374
  Entrez Gene 4759 Human
  UniProt ID Q9Y3R4
• Background: This gene belongs to a family of glycohydrolytic enzymes which remove sialic acid residues from glycoproteins and glycolipids. Expression studies in COS7 cells confirmed that this gene encodes a functional sialidase. Its cytosolic localization was demonstrated by cell fractionation experiments. [provided by RefSeq]
• Synonyms: SIAL2

Reference Data

• Protein Pathways: Other glycan degradation, Sphingolipid metabolism