FGF21 Mouse Monoclonal Antibody [Clone ID: OTI1H4]

CAT#: CF502953

Carrier-free (BSA/glycerol-free) FGF21 mouse monoclonal antibody, clone OTI1H4 (formerly 1H4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FGF21 (RC204538, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FGF21. Positive lysates LY412740 (100ug) and LC412740 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (10ug) from 1 cell line by using anti-FGF21 monoclonal antibody at 1:200.

• 

  Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-FGF21 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).

• 

  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-FGF21 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502953)

• 

  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-FGF21 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502953)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-FGF21 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502953)

• Western blot analysis of extracts (10ug) from Hela cell line by using anti-FGF21 monoclonal antibody at 1:200.

• HEK293T cells transfected with either RC204538 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FGF21 antibody (TA502953), and then analyzed by flow cytometry.

Show all 8 images

Specifications

Product Data

• Clone Name: OTI1H4
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:200 - 1:1000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human FGF21(NP_061986) produced in HEK293 cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 19.4 kDa
• Gene Name: Homo sapiens fibroblast growth factor 21 (FGF21), mRNA.
• Database Link:
  NP_061986
  Entrez Gene 26291 Human
  UniProt ID Q9NSA1
• Background: The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The function of this growth factor has not yet been determined. [provided by RefSeq]
• Synonyms: fibroblast growth factor 21

Reference Data

• Protein Families: Adult stem cells, Embryonic stem cells, ES Cell Differentiation/IPS, Secreted Protein
• Protein Pathways: MAPK signaling pathway, Melanoma, Pathways in cancer, Regulation of actin cytoskeleton