EB2 (MAPRE2) Mouse Monoclonal Antibody [Clone ID: OTI5F3]

CAT#: CF502961

Carrier-free (BSA/glycerol-free) MAPRE2 mouse monoclonal antibody, clone OTI5F3 (formerly 5F3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPRE2 (RC200259, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPRE2. Positive lysates LY415395 (100ug) and LC415395 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MAPRE2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502961)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MAPRE2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502961)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MAPRE2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502961)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MAPRE2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502961)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAPRE2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502961)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-MAPRE2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502961)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-MAPRE2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502961)

• Anti-MAPRE2 mouse monoclonal antibody (TA502961) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPRE2 (RC200259 ).

• HEK293T cells transfected with either RC200259 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAPRE2 antibody (TA502961), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-MAPRE2 antibody (TA502961), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-MAPRE2 antibody (TA502961), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI5F3
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MAPRE2 (NP_055083) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 36.9 kDa
• Gene Name: Homo sapiens microtubule associated protein RP/EB family member 2 (MAPRE2), transcript variant 1, mRNA.
• Database Link:
  NP_055083
  Entrez Gene 10982 Human
  UniProt ID Q15555
• Background: The protein encoded by this gene shares significant homology to the adenomatous polyposis coli (APC) protein-binding EB1 gene family. This protein is a microtubule-associated protein that is necessary for spindle symmetry during mitosis. It is thought to play a role in the tumorigenesis of colorectal cancers and the proliferative control of normal cells. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2012]
• Synonyms: EB1; EB2; RP1

Reference Data

• Protein Families: Druggable Genome