SNAP25 Mouse Monoclonal Antibody [Clone ID: OTI4B2]

CAT#: CF502968

Carrier-free (BSA/glycerol-free) SNAP25 mouse monoclonal antibody, clone OTI4B2 (formerly 4B2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNAP25 (RC202068, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNAP25. Positive lysates LY418912 (100ug) and LC418912 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-SNAP25 monoclonal antibody (1:200).

• 

  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SNAP25 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502968)

• 

  Anti-SNAP25 mouse monoclonal antibody (TA502968) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SNAP25 (RC202068).

• 

  HEK293T cells transfected with either RC202068 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SNAP25 antibody (TA502968), and then analyzed by flow cytometry.

Show all 5 images

Specifications

Product Data

• Clone Name: OTI4B2
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SNAP25(NP_003072) produced in HEK293 cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 23.2 kDa
• Gene Name: Homo sapiens synaptosome associated protein 25 (SNAP25), transcript variant 1, mRNA.
• Database Link:
  NP_003072
  Entrez Gene 20614 MouseEntrez Gene 25012 RatEntrez Gene 6616 Human
  UniProt ID P60880
• Background: Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq]
• Synonyms: bA416N4.2; dJ1068F16.2; RIC-4; RIC4; SEC9; SNAP; SNAP-25

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: SNARE interactions in vesicular transport