LIM Kinase 1 (LIMK1) Mouse Monoclonal Antibody [Clone ID: OTI6A7]
CAT#: CF502974
Carrier-free (BSA/glycerol-free) LIMK1 mouse monoclonal antibody, clone OTI6A7 (formerly 6A7)
Formulation: Standard
Other products for "LIMK1"
Specifications
Product Data | |
Clone Name | OTI6A7 |
Applications | FC, IF, WB |
Recommended Dilution | WB 1:2000, IF 1:100, FLOW 1:100 |
Reactivities | Human, Mouse, Rat |
Host | Mouse |
Isotype | IgG2a |
Clonality | Monoclonal |
Immunogen | Full length human recombinant protein of human LIMK1(NP_002305) produced in HEK293 cell. |
Formulation | Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose) |
Reconstitution Method | For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific) |
Purification | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
Conjugation | Unconjugated |
Storage | Store at -20°C as received. |
Stability | Stable for 12 months from date of receipt. |
Predicted Protein Size | 72.4 kDa |
Gene Name | Homo sapiens LIM domain kinase 1 (LIMK1), transcript variant 1, mRNA. |
Database Link | |
Background | There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
Synonyms | LIMK; LIMK-1 |
Reference Data | |
Protein Families | Druggable Genome, Protein Kinase |
Protein Pathways | Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton |
Documents
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