BAIAP2 Mouse Monoclonal Antibody [Clone ID: OTI 1D9]

CAT#: CF502981

Carrier-free (BSA/glycerol-free) BAIAP2 mouse monoclonal antibody, clone OTI 1D9 (formerly 1D9)

Product Images

Anti-BAIAP2 mouse monoclonal antibody (TA502981) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BAIAP2 (RC214570).

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BAIAP2 (RC214570, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BAIAP2. Positive lysates LY401909 (100ug) and LC401909 (20ug) can be purchased separately from OriGene.

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  Flow cytometric Analysis of Hela cells, using anti-BAIAP2 antibody (TA502981), (Red), compared to a nonspecific negative control antibody, (Blue).

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  HEK293T cells transfected with either RC214570 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BAIAP2 antibody (TA502981), and then analyzed by flow cytometry.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-BAIAP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502981)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-BAIAP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502981)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-BAIAP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502981)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-BAIAP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502981)

• Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BAIAP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502981)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-BAIAP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502981)

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Specifications

Product Data

• Clone Name: OTI 1D9
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human BAIAP2 (NP_006331) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 57.3 kDa
• Gene Name: Homo sapiens BAI1 associated protein 2 (BAIAP2), transcript variant 3, mRNA.
• Database Link:
  NP_006331
  Entrez Gene 108100 MouseEntrez Gene 117542 RatEntrez Gene 10458 Human
  UniProt ID Q9UQB8
• Background: The protein encoded by this gene has been identified as a brain-specific angiogenesis inhibitor (BAI1)-binding protein. This adaptor protein links membrane bound G-proteins to cytoplasmic effector proteins. This protein functions as an insulin receptor tyrosine kinase substrate and suggests a role for insulin in the central nervous system. It also associates with a downstream effector of Rho small G proteins, which is associated with the formation of stress fibers and cytokinesis. This protein is involved in lamellipodia and filopodia formation in motile cells and may affect neuronal growth-cone guidance. This protein has also been identified as interacting with the dentatorubral-pallidoluysian atrophy gene, which is associated with an autosomal dominant neurodegenerative disease. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jan 2009]
• Synonyms: BAP2; FLAF3; IRSP53

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Regulation of actin cytoskeleton