beta IV Tubulin (TUBB4A) Mouse Monoclonal Antibody [Clone ID: OTI3F1]

CAT#: CF502985

Carrier-free (BSA/glycerol-free) TUBB4 mouse monoclonal antibody, clone OTI3F1 (formerly 3F1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TUBB4 (RC203945, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TUBB4. Positive lysates LY401833 (100ug) and LC401833 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TUBB4 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TUBB4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502985)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TUBB4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502985)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TUBB4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502985)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TUBB4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502985)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TUBB4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502985)

• Anti-TUBB4 mouse monoclonal antibody (TA502985) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TUBB4 (RC203945).

• Flow cytometric Analysis of Hela cells, using anti-TUBB4 antibody (TA502985), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-TUBB4 antibody (TA502985), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI3F1
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TUBB4 (NP_006078) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 49.4 kDa
• Gene Name: Homo sapiens tubulin beta 4A class IVa (TUBB4A), transcript variant 3, mRNA.
• Database Link:
  NP_006078
  Entrez Gene 22153 MouseEntrez Gene 29213 RatEntrez Gene 100855815 DogEntrez Gene 703019 MonkeyEntrez Gene 10382 Human
  UniProt ID P04350
• Synonyms: beta-5; DYT4; TUBB4

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Gap junction, Pathogenic Escherichia coli infection