RGS5 Mouse Monoclonal Antibody [Clone ID: OTI1C1]

CAT#: CF503075

Carrier-free (BSA/glycerol-free) RGS5 mouse monoclonal antibody, clone OTI1C1 (formerly 1C1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RGS5 (RC206857, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RGS5. Positive lysates LY418548 (100ug) and LC418548 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RGS5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503075)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-RGS5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503075)

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  Figure from citation: Immunofluorescence of RGS5 protein level by using anti-RGS5 antibody in primary neonatal pig GMCs. View Citation

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  Figure from citation: Immunofluorescence of RGS5 protein level by using anti-RGS5 antibody in human brain pericytes. Dilution: 1:200 View Citation

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  HEK293T cells transfected with either RC206857 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RGS5 antibody (TA503075), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Jurkat cells, using anti-RGS5 antibody (TA503075), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI1C1
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RGS5 (NP_003608) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 20.8 kDa
• Gene Name: Homo sapiens regulator of G protein signaling 5 (RGS5), transcript variant 1, mRNA.
• Database Link:
  NP_003608
  Entrez Gene 19737 MouseEntrez Gene 54294 RatEntrez Gene 8490 Human
  UniProt ID O15539
• Background: This gene encodes a member of the regulators of G protein signaling (RGS) family. The RGS proteins are signal transduction molecules which are involved in the regulation of heterotrimeric G proteins by acting as GTPase activators. This gene is a hypoxia-inducible factor-1 dependent, hypoxia-induced gene which is involved in the induction of endothelial apoptosis. This gene is also one of three genes on chromosome 1q contributing to elevated blood pressure. Alternatively spliced transcript variants encoding different isoforms have been identified.
• Synonyms: MST092; MST106; MST129; MSTP032; MSTP092; MSTP106; MSTP129

Reference Data

• Protein Families: Druggable Genome