LSM1 Mouse Monoclonal Antibody [Clone ID: OTI5C6]

CAT#: CF503120

Carrier-free (BSA/glycerol-free) LSM1 mouse monoclonal antibody, clone OTI5C6 (formerly 5C6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY LSM1 (Cat# RC200288, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LSM1(Cat# TA503120). Positive lysates LY415265 (100ug) and LC415265 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-LSM1 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-LSM1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LSM1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-LSM1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-LSM1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Anti-LSM1 mouse monoclonal antibody (TA503120) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LSM1 (RC200288).

• HEK293T cells transfected with either RC200288 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LSM1 antibody (TA503120), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-LSM1 antibody (TA503120), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-LSM1 antibody (TA503120), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI5C6
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Dog, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human LSM1 (NP_055277) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 15 kDa
• Gene Name: LSM1 homolog, mRNA degradation associated
• Database Link:
  NP_055277
  Entrez Gene 67207 MouseEntrez Gene 364624 RatEntrez Gene 475587 DogEntrez Gene 27257 Human
  UniProt ID O15116
• Background: Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family (see SNRPD2; MIM 601061). Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing. [supplied by OMIM]
• Synonyms: CASM; YJL124C

Reference Data

• Protein Families: Stem cell - Pluripotency
• Protein Pathways: RNA degradation