GATM Mouse Monoclonal Antibody [Clone ID: OTI1C9]

CAT#: CF503147

Carrier-free (BSA/glycerol-free) GATM mouse monoclonal antibody, clone OTI1C9 (formerly 1C9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GATM (RC200474, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GATM. Positive lysates LY419923 (100ug) and LC419923 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-GATM monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-GATM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503147)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-GATM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503147)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-GATM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503147)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-GATM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503147)

• Anti-GATM mouse monoclonal antibody (TA503147) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GATM (RC200474).

• HEK293T cells transfected with either RC200474 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GATM antibody (TA503147), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1C9
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GATM(NP_001473) produced in HEK293 cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 44.2 kDa
• Gene Name: Homo sapiens glycine amidinotransferase (GATM), transcript variant 1, mRNA.
• Database Link:
  NP_001473
  Entrez Gene 67092 MouseEntrez Gene 81660 RatEntrez Gene 2628 Human
  UniProt ID P50440
• Background: This gene encodes a mitochondrial enzyme that belongs to the amidinotransferase family. This enzyme is involved in creatine biosynthesis, whereby it catalyzes the transfer of a guanido group from L-arginine to glycine, resulting in guanidinoacetic acid, the immediate precursor of creatine. Mutations in this gene cause arginine:glycine amidinotransferase deficiency, an inborn error of creatine synthesis characterized by mental retardation, language impairment, and behavioral disorders. [provided by RefSeq]
• Synonyms: AGAT; AT; CCDS3

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Arginine and proline metabolism, Glycine, serine and threonine metabolism, Metabolic pathways