GPR48 (LGR4) Mouse Monoclonal Antibody [Clone ID: OTI5B8]

CAT#: CF503215

Carrier-free (BSA/glycerol-free) LGR4 mouse monoclonal antibody, clone OTI5B8 (formerly 5B8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGR4 (RC221345, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGR4. Positive lysates LY413017 (100ug) and LC413017 (20ug) can be purchased separately from OriGene.

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  HEK293T cells transfected with either RC221345 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LGR4 antibody (TA503215), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI5B8
• Applications: FC, WB
• Recommended Dilution: WB 1:500, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 230-540 of human LGR4 (NP_060960 ) produced in HEK293T Cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens leucine rich repeat containing G protein-coupled receptor 4 (LGR4), transcript variant 1, mRNA.
• Database Link:
  NP_060960
  Entrez Gene 107515 MouseEntrez Gene 286994 RatEntrez Gene 55366 Human
  UniProt ID Q9BXB1
• Background: G protein-coupled receptors (GPCRs) play key roles in a variety of physiologic functions. Members of the leucine-rich GPCR (LGR) family, such as GPR48, have multiple N-terminal leucine-rich repeats (LRRs) and a 7-transmembrane domain (Weng et al., 2008 [PubMed 18424556]). [supplied by OMIM]
• Synonyms: BNMD17; GPR48

Reference Data

• Protein Families: Druggable Genome, GPCR, Transmembrane