XPF (ERCC4) Mouse Monoclonal Antibody [Clone ID: OTI4E11]

CAT#: CF503261

Carrier-free (BSA/glycerol-free) ERCC4 mouse monoclonal antibody, clone OTI4E11 (formerly 4E11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC4 (RC223300, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC4. Positive lysates LY401605 (100ug) and LC401605 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ERCC4 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ERCC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503261)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ERCC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503261)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ERCC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503261)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ERCC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503261)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ERCC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503261)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ERCC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503261)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ERCC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503261)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ERCC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503261)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ERCC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503261)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ERCC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503261)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ERCC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503261)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ERCC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503261)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-ERCC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503261)

• Anti-ERCC4 mouse monoclonal antibody (TA503261) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC4 (RC223300).

• HEK293T cells transfected with either RC223300 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ERCC4 antibody (TA503261), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI4E11
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ERCC4(NP_005227) produced in HEK293 cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 104.3 kDa
• Gene Name: Homo sapiens ERCC excision repair 4, endonuclease catalytic subunit (ERCC4), mRNA.
• Database Link:
  NP_005227
  Entrez Gene 50505 MouseEntrez Gene 2072 Human
  UniProt ID Q92889
• Background: The protein encoded by this gene forms a complex with ERCC1 and is involved in the 5' incision made during nucleotide excision repair. This complex is a structure specific DNA repair endonuclease that interacts with EME1. Defects in this gene are a cause of xeroderma pigmentosum complementation group F (XP-F), or xeroderma pigmentosum VI (XP6).
• Synonyms: ERCC11; FANCQ; RAD1; XFEPS; XPF

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Nucleotide excision repair