SIRT5 Mouse Monoclonal Antibody [Clone ID: OTI2E10]

CAT#: CF503283

Carrier-free (BSA/glycerol-free) SIRT5 mouse monoclonal antibody, clone OTI2E10 (formerly 2E10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIRT5 (RC200189, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIRT5. Positive lysates LY415885 (100ug) and LC415885 (20ug) can be purchased separately from OriGene.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and SIRT5-Knockout HeLa cells (KO, Cat# LC830483) were separated by SDS-PAGE and immunoblotted with anti-SIRT5 monoclonal antibody TA503283 (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

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  HEK293T cells transfected with either RC200189 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SIRT5 antibody (TA503283), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI2E10
• Applications: FC, WB
• Recommended Dilution: WB 1:2000, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SIRT5 (NP_036373) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.7 kDa
• Gene Name: Homo sapiens sirtuin 5 (SIRT5), transcript variant 1, mRNA.
• Database Link:
  NP_036373
  Entrez Gene 68346 MouseEntrez Gene 306840 RatEntrez Gene 23408 Human
  UniProt ID Q9NXA8
• Background: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq]
• Synonyms: SIR2L5

Reference Data

• Protein Families: Druggable Genome, Transcription Factors