TAZ (WWTR1) Mouse Monoclonal Antibody [Clone ID: OTI1B5]

CAT#: CF503396

Carrier-free (BSA/glycerol-free) WWTR1 mouse monoclonal antibody, clone OTI1B5 (formerly 1B5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WWTR1 (RC204082, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WWTR1. Positive lysates LY414524 (100ug) and LC414524 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 1 cell line by using anti-WWTR1 monoclonal antibody (1:200).

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  Anti-WWTR1 mouse monoclonal antibody (TA503396) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY WWTR1 (RC204082).

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  HEK293T cells transfected with either RC204082 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-WWTR1 antibody (TA503396), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1B5
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:200~500, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human WWTR1(NP_056287) produced in HEK293 cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43.9 kDa
• Gene Name: Homo sapiens WW domain containing transcription regulator 1 (WWTR1), transcript variant 1, mRNA.
• Database Link:
  NP_056287
  Entrez Gene 97064 MouseEntrez Gene 295062 RatEntrez Gene 25937 Human
  UniProt ID Q9GZV5
• Synonyms: TAZ

Reference Data

• Protein Families: Druggable Genome, Transcription Factors