Apc2 (ANAPC2) Mouse Monoclonal Antibody [Clone ID: OTI1H8]

CAT#: CF503407

Carrier-free (BSA/glycerol-free) ANAPC2 mouse monoclonal antibody, clone OTI1H8 (formerly 1H8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANAPC2 (RC207539, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANAPC2. Positive lysates LY402247 (100ug) and LC402247 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ANAPC2 monoclonal antibody.

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  Anti-ANAPC2 mouse monoclonal antibody (TA503407) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ANAPC2 (RC207539).

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  HEK293T cells transfected with either RC207539 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ANAPC2 antibody (TA503407), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1H8
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500~2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ANAPC2(NP_037498) produced in HEk293 cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 93.6 kDa
• Gene Name: Homo sapiens anaphase promoting complex subunit 2 (ANAPC2), mRNA.
• Database Link:
  NP_037498
  Entrez Gene 99152 MouseEntrez Gene 296558 RatEntrez Gene 29882 Human
  UniProt ID Q9UJX6
• Background: A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The product of this gene is a component of the complex and shares sequence similarity with a recently identified family of proteins called cullins, which may also be involved in ubiquitin-mediated degradation. [provided by RefSeq]
• Synonyms: APC2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis