LGALS3BP Mouse Monoclonal Antibody [Clone ID: OTI1F8]

CAT#: CF503454

Carrier-free (BSA/glycerol-free) LGALS3BP mouse monoclonal antibody, clone OTI1F8 (formerly 1F8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGALS3BP (RC204918, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGALS3BP. Positive lysates LY417206 (100ug) and LC417206 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-LGALS3BP monoclonal antibody (1:200).

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  Anti-LGALS3BP mouse monoclonal antibody (TA503454) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LGALS3BP (RC204918).

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  HEK293T cells transfected with either RC204918 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LGALS3BP antibody (TA503454), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1F8
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment cprresponding to amino acids 19-300 of human LGALS3BP(NP_005558) produced in E.coil.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 65.2 kDa
• Gene Name: Homo sapiens galectin 3 binding protein (LGALS3BP), mRNA.
• Database Link:
  NP_005558
  Entrez Gene 3959 Human
  UniProt ID Q08380
• Background: The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS3BP has been found elevated in the serum of patients with cancer and in those infected by the human immunodeficiency virus (HIV). It appears to be implicated in immune response associated with natural killer (NK) and lymphokine-activated killer (LAK) cell cytotoxicity. Using fluorescence in situ hybridization the full length 90K cDNA has been localized to chromosome 17q25. The native protein binds specifically to a human macrophage-associated lectin known as Mac-2 and also binds galectin 1. [provided by RefSeq, Jul 2008]
• Synonyms: 90K; BTBD17B; CyCAP; gp90; M2BP; MAC-2-BP; TANGO10B

Reference Data

• Protein Families: Druggable Genome, Secreted Protein