FDFT1 Mouse Monoclonal Antibody [Clone ID: OTI2F10]

CAT#: CF503470

Carrier-free (BSA/glycerol-free) FDFT1 mouse monoclonal antibody, clone OTI2F10 (formerly 2F10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FDFT1 (RC201392, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FDFT1. Positive lysates LY401419 (100ug) and LC401419 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FDFT1 monoclonal antibody.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and FDFT1-Knockout 293T cells (KO, Cat# LC841996) were separated by SDS-PAGE and immunoblotted with anti-FDFT1 monoclonal antibody TA503470, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-FDFT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503470)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-FDFT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503470)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-FDFT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503470)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-FDFT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503470)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-FDFT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503470)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-FDFT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503470)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-FDFT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503470)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-FDFT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503470)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-FDFT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503470)

• Anti-FDFT1 mouse monoclonal antibody (TA503470) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FDFT1 (RC201392).

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Specifications

Product Data

• Clone Name: OTI2F10
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:200~500, IHC 1:150, IF 1:100
• Reactivities: Human, Monkey, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human FDFT1(NP_004453) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 47.9 kDa
• Gene Name: Homo sapiens farnesyl-diphosphate farnesyltransferase 1 (FDFT1), transcript variant 3, mRNA.
• Database Link:
  NP_004453
  Entrez Gene 14137 MouseEntrez Gene 29580 RatEntrez Gene 696396 MonkeyEntrez Gene 2222 Human
  UniProt ID P37268
• Background: This gene encodes a membrane-associated enzyme located at a branch point in the mevalonate pathway. The encoded protein is the first specific enzyme in cholesterol biosynthesis, catalyzing the dimerization of two molecules of farnesyl diphosphate in a two-step reaction to form squalene. [provided by RefSeq]
• Synonyms: DGPT; ERG9; SQS; SS

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Steroid biosynthesis