Her2 (ERBB2) Mouse Monoclonal Antibody [Clone ID: OTI3F11]

CAT#: CF503525

Carrier-free (BSA/glycerol-free) ERBB2 mouse monoclonal antibody, clone OTI3F11 (formerly 3F11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERBB2 (RC212583, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERBB2. Positive lysates LY417979 (100ug) and LC417979 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-ERBB2 monoclonal antibody (1:500).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and ERBB2-Knockout Hela cells (KO, Cat# LC810070) were separated by SDS-PAGE and immunoblotted with anti-ERBB2 monoclonal antibody TA503525. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control (1:200).

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ERBB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503525)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ERBB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503525)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ERBB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503525)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ERBB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503525)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ERBB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503525)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-ERBB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503525)

• Anti-ERBB2 mouse monoclonal antibody (TA503525) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERBB2 (RC212583).

• HEK293T cells transfected with either RC212583 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ERBB2 antibody (TA503525), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Jurkat cells, using anti-ERBB2 antibody (TA503525), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI3F11
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 676-1255 of human ERBB2(NP_004439) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 137.7 kDa
• Gene Name: Homo sapiens erb-b2 receptor tyrosine kinase 2 (ERBB2), transcript variant 1, mRNA.
• Database Link:
  NP_004439
  Entrez Gene 13866 MouseEntrez Gene 24337 RatEntrez Gene 2064 Human
  UniProt ID P04626
• Background: This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq]
• Synonyms: CD340; HER-2; HER2; MLN 19; NEU; neu; NGL; TKR1

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Adherens junction, Bladder cancer, Calcium signaling pathway, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer