PPAR alpha (PPARA) Mouse Monoclonal Antibody [Clone ID: OTI2F3]

CAT#: CF503542

Carrier-free (BSA/glycerol-free) PPARA mouse monoclonal antibody, clone OTI2F3 (formerly 2F3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPARA (RC216176, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPARA. Positive lysates LY401560 (100ug) and LC401560 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 1 cell line by using anti-PPARA monoclonal antibody (1:200).

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  Anti-PPARA mouse monoclonal antibody (TA503542) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPARA (RC216176).

Specifications

Product Data

• Clone Name: OTI2F3
• Applications: IF, WB
• Recommended Dilution: WB 1:500, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 145-420 of human PPARA(NP_005027) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 52 kDa
• Gene Name: Homo sapiens peroxisome proliferator activated receptor alpha (PPARA), transcript variant 5, mRNA.
• Database Link:
  NP_005027
  Entrez Gene 19013 MouseEntrez Gene 25747 RatEntrez Gene 5465 Human
  UniProt ID Q07869
• Background: Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. [provided by RefSeq, Jul 2008]
• Synonyms: hPPAR; NR1C1; PPAR; PPARalpha

Reference Data

• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
• Protein Pathways: Adipocytokine signaling pathway, PPAR signaling pathway