Rad51L1 (RAD51B) Mouse Monoclonal Antibody [Clone ID: OTI7H4]

CAT#: CF503550

Carrier-free (BSA/glycerol-free) RAD51L1 mouse monoclonal antibody, clone OTI7H4 (formerly 7H4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAD51L1 (RC206457, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAD51L1. Positive lysates LY408768 (100ug) and LC408768 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-RAD51L1 monoclonal antibody at 1:200 dilution.

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  Anti-RAD51L1 mouse monoclonal antibody (TA503550) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RAD51L1 (RC206457).

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  HEK293T cells transfected with either RC206457 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RAD51L1 antibody (TA503550), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI7H4
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 119-346 of human RAD51L1(NP_598193) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 42 kDa
• Gene Name: Homo sapiens RAD51 paralog B (RAD51B), transcript variant 3, mRNA.
• Database Link:
  NP_598193
  Entrez Gene 19363 MouseEntrez Gene 5890 Human
  UniProt ID O15315
• Background: The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are evolutionarily conserved proteins essential for DNA repair by homologous recombination. This protein has been shown to form a stable heterodimer with the family member RAD51C, which further interacts with the other family members, such as RAD51, XRCC2, and XRCC3. Overexpression of this gene was found to cause cell cycle G1 delay and cell apoptosis, which suggested a role of this protein in sensing DNA damage. At least three alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]
• Synonyms: R51H2; RAD51L1; REC2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Homologous recombination