SEC14 like protein 2 (SEC14L2) Mouse Monoclonal Antibody [Clone ID: OTI1H3]

CAT#: CF503561

Carrier-free (BSA/glycerol-free) SEC14L2 mouse monoclonal antibody, clone OTI1H3 (formerly 1H3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SEC14L2 (RC215994, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SEC14L2. Positive lysates LY415756 (100ug) and LC415756 (20ug) can be purchased separately from OriGene.

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  Anti-SEC14L2 mouse monoclonal antibody (TA503561) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SEC14L2 (RC215994).

Specifications

Product Data

• Clone Name: OTI1H3
• Applications: IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SEC14L2(NP_036561) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46 kDa
• Gene Name: Homo sapiens SEC14 like lipid binding 2 (SEC14L2), transcript variant 1, mRNA.
• Database Link:
  NP_036561
  Entrez Gene 67815 MouseEntrez Gene 23541 Human
  UniProt ID O76054
• Background: This gene encodes a cytosolic protein which belongs to a family of lipid-binding proteins including Sec14p, alpha-tocopherol transfer protein, and cellular retinol-binding protein. The encoded protein stimulates squalene monooxygenase which is a downstream enzyme in the cholesterol biosynthetic pathway. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Oct 2008]
• Synonyms: C22orf6; SPF; TAP; TAP1

Reference Data

• Protein Families: Transcription Factors