EIF2B3 Mouse Monoclonal Antibody [Clone ID: OTI1H3]

CAT#: CF503562

Carrier-free (BSA/glycerol-free) EIF2B3 mouse monoclonal antibody, clone OTI1H3 (formerly 1H3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF2B3 (RC223570, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF2B3. Positive lysates LY412535 (100ug) and LC412535 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 5 different cell lines by using anti-EIF2B3 monoclonal antibody (1:200).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-EIF2B3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503562)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-EIF2B3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503562)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-EIF2B3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503562)

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  Anti-EIF2B3 mouse monoclonal antibody (TA503562) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY EIF2B3 (RC223570).

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Specifications

Product Data

• Clone Name: OTI1H3
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human EIF2B3(NP_065098) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50.1 kDa
• Gene Name: Homo sapiens eukaryotic translation initiation factor 2B subunit gamma (EIF2B3), transcript variant 1, mRNA.
• Database Link:
  NP_065098
  Entrez Gene 108067 MouseEntrez Gene 171145 RatEntrez Gene 8891 Human
  UniProt ID Q9NR50
• Background: The protein encoded by this gene is one of the subunits of initiation factor eIF2B, which catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. It has also been found to function as a cofactor of hepatitis C virus internal ribosome entry site-mediated translation. Mutations in this gene have been associated with leukodystrophy with vanishing white matter. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
• Synonyms: EIF-2B; EIF2Bgamma