UNG Mouse Monoclonal Antibody [Clone ID: OTI2C12]

CAT#: CF503563

Carrier-free (BSA/glycerol-free) UNG mouse monoclonal antibody, clone OTI2C12 (formerly 2C12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UNG (RC222868, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UNG. Positive lysates LY408998 (100ug) and LC408998 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-UNG monoclonal antibody.

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  Western blot analysis of extracts (10ug) from a mouse tissue by using anti-UNG monoclonal antibody (1:200).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and UNG-Knockout HeLa cells (KO, Cat# LC830875) were separated by SDS-PAGE and immunoblotted with anti-UNG monoclonal antibody TA503563 (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-UNG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503563)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-UNG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503563)

• Anti-UNG mouse monoclonal antibody (TA503563) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY UNG (RC222868).

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Specifications

Product Data

• Clone Name: OTI2C12
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human UNG(NP_550433) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 34.5 kDa
• Gene Name: Homo sapiens uracil DNA glycosylase (UNG), transcript variant 2, mRNA.
• Database Link:
  NP_550433
  Entrez Gene 22256 MouseEntrez Gene 304577 RatEntrez Gene 7374 Human
  UniProt ID P13051
• Background: This gene encodes one of several uracil-DNA glycosylases. One important function of uracil-DNA glycosylases is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. Uracil bases occur from cytosine deamination or misincorporation of dUMP residues. Alternative promoter usage and splicing of this gene leads to two different isoforms: the mitochondrial UNG1 and the nuclear UNG2. The UNG2 term was used as a previous symbol for the CCNO gene (GeneID 10309), which has been confused with this gene, in the literature and some databases. [provided by RefSeq]
• Synonyms: DGU; HIGM4; HIGM5; UDG; UNG1; UNG2; UNG15

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Base excision repair, Primary immunodeficiency