ELK3 Mouse Monoclonal Antibody [Clone ID: OTI1H3]

CAT#: CF503603

Carrier-free (BSA/glycerol-free) ELK3 mouse monoclonal antibody, clone OTI1H3 (formerly 1H3)

Product Images

HEK293T cells transfected with either RC203114 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ELK3 antibody (TA503603), and then analyzed by flow cytometry.

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  Anti-ELK3 mouse monoclonal antibody (TA503603) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ELK3 (RC203114).

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ELK3 (RC203114, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ELK3. Positive lysates LY401602 (100ug) and LC401602 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-ELK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503603)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ELK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503603)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ELK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503603)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ELK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503603)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ELK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503603)

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Specifications

Product Data

• Clone Name: OTI1H3
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ELK3(NP_005221) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 44.1 kDa
• Gene Name: Homo sapiens ETS transcription factor ELK3 (ELK3), transcript variant 1, mRNA.
• Database Link:
  NP_005221
  Entrez Gene 13713 MouseEntrez Gene 2004 Human
  UniProt ID P41970
• Background: The protein encoded by this gene is a member of the ETS-domain transcription factor family and the ternary complex factor (TCF) subfamily. Proteins in this subfamily regulate transcription when recruited by serum response factor to bind to serum response elements. This protein is activated by signal-induced phosphorylation; studies in rodents suggest that it is a transcriptional inhibitor in the absence of Ras, but activates transcription when Ras is present. [provided by RefSeq]
• Synonyms: ERP; NET; SAP-2; SAP2

Reference Data

• Protein Families: Transcription Factors