TMED1 Mouse Monoclonal Antibody [Clone ID: OTI7G4]

CAT#: CF503659

Carrier-free (BSA/glycerol-free) TMED1 mouse monoclonal antibody, clone OTI7G4 (formerly 7G4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TMED1 (RC200255, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TMED1. Positive lysates LY402049 (100ug) and LC402049 (20ug) can be purchased separately from OriGene.

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  HEK293T cells transfected with either RC200255 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TMED1 antibody (TA503659), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI7G4
• Applications: FC, WB
• Recommended Dilution: WB 1:2000, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TMED1(NP_006849) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 22.8 kDa
• Gene Name: Homo sapiens transmembrane p24 trafficking protein 1 (TMED1), transcript variant 1, mRNA.
• Database Link:
  NP_006849
  Entrez Gene 17083 MouseEntrez Gene 315461 RatEntrez Gene 11018 Human
  UniProt ID Q13445
• Background: The protein encoded by this gene was identified by its interaction with interleukin 1 receptor-like 1 (IL1RL1). This protein lacks any similarity to other interleukin 1 ligands. The functional significance of its interaction with IL1RL1 is not known. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: Il1rl1l; IL1RL1LG; p24g1; Tp24

Reference Data

• Protein Families: Druggable Genome, Transmembrane