Acetyl CoA synthetase (ACSS2) Mouse Monoclonal Antibody [Clone ID: OTI2E9]

CAT#: CF503684

Carrier-free (BSA/glycerol-free) ACSS2 mouse monoclonal antibody, clone OTI2E9 (formerly 2E9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACSS2 (RC204260, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACSS2. Positive lysates LY412981 (100ug) and LC412981 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI2E9
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ACSS2(NP_061147) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 78.4 kDa
• Gene Name: Homo sapiens acyl-CoA synthetase short chain family member 2 (ACSS2), transcript variant 1, mRNA.
• Database Link:
  NP_061147
  Entrez Gene 60525 MouseEntrez Gene 311569 RatEntrez Gene 55902 Human
  UniProt ID Q9NR19
• Background: This gene encodes a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Alternative splicing results in multiple transcript variants. [provided by RefSeq]
• Synonyms: ACAS2; ACECS; ACS; ACSA; dJ1161H23.1

Reference Data

• Protein Pathways: Glycolysis / Gluconeogenesis, Metabolic pathways, Propanoate metabolism, Pyruvate metabolism