SCDGFB (PDGFD) Mouse Monoclonal Antibody [Clone ID: OTI1C1]

CAT#: CF503700

Carrier-free (BSA/glycerol-free) PDGFD mouse monoclonal antibody, clone OTI1C1 (formerly 1C1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDGFD (RC219641, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDGFD. Positive lysates LY403061 (100ug) and LC403061 (20ug) can be purchased separately from OriGene.

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  HEK293T cells transfected with either RC219641 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PDGFD antibody (TA503700), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1C1
• Applications: FC, WB
• Recommended Dilution: WB 1:2000, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 19-247 of human PDGFD(NP_079484) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 40.2 kDa
• Gene Name: Homo sapiens platelet derived growth factor D (PDGFD), transcript variant 1, mRNA.
• Database Link:
  NP_079484
  Entrez Gene 66018 RatEntrez Gene 71785 MouseEntrez Gene 80310 Human
  UniProt ID Q9GZP0
• Background: The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines, seven of which are found in this factor. This gene product only forms homodimers and, therefore, does not dimerize with the other three family members. It differs from alpha and beta members of this family in having an unusual N-terminal domain, the CUB domain. Two splice variants have been identified for this gene. [provided by RefSeq]
• Synonyms: IEGF; MSTP036; SCDGF-B; SCDGFB

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Focal adhesion, Gap junction, Melanoma, Prostate cancer, Regulation of actin cytoskeleton