UBE2M Mouse Monoclonal Antibody [Clone ID: OTI2D9]

CAT#: CF503770

Carrier-free (BSA/glycerol-free) UBE2M mouse monoclonal antibody, clone OTI2D9 (formerly 2D9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2M (RC208946, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2M. Positive lysates LY418316 (100ug) and LC418316 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-UBE2M monoclonal antibody.

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  Anti-UBE2M mouse monoclonal antibody (TA503770) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY UBE2M (RC208946).

Specifications

Product Data

• Clone Name: OTI2D9
• Applications: IF, WB
• Recommended Dilution: WB 1:500~2000, IF 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human UBE2M(NP_003960) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 20.7 kDa
• Gene Name: Homo sapiens ubiquitin conjugating enzyme E2 M (UBE2M), mRNA.
• Database Link:
  NP_003960
  Entrez Gene 22192 MouseEntrez Gene 361509 RatEntrez Gene 484221 DogEntrez Gene 712206 MonkeyEntrez Gene 9040 Human
  UniProt ID P61081
• Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. [provided by RefSeq]
• Synonyms: hUbc12; UBC-RS2; UBC12

Reference Data

• Protein Pathways: Ubiquitin mediated proteolysis