Lipoprotein lipase (LPL) Mouse Monoclonal Antibody [Clone ID: OTI3A10]

CAT#: CF503793

Carrier-free (BSA/glycerol-free) LPL mouse monoclonal antibody, clone OTI3A10 (formerly 3A10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY LPL (Cat# RC203766, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LPL (Cat# TA503793). Positive lysates LY400089 (100ug) and LC400089 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-LPL monoclonal antibody (1:200).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-LPL mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-LPL mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-LPL mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-LPL mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• HEK293T cells transfected with either RC203766 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LPL antibody (TA503793), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI3A10
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 28-475 of human LPL(NP_000228) produced in HEK293T.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50.3 kDa
• Gene Name: lipoprotein lipase
• Database Link:
  NP_000228
  Entrez Gene 16956 MouseEntrez Gene 24539 RatEntrez Gene 4023 Human
  UniProt ID P06858
• Background: LPL encodes lipoprotein lipase, which is expressed in heart, muscle, and adipose tissue. LPL functions as a homodimer, and has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. Severe mutations that cause LPL deficiency result in type I hyperlipoproteinemia, while less extreme mutations in LPL are linked to many disorders of lipoprotein metabolism. [provided by RefSeq]
• Synonyms: HDLCQ11; LIPD

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Glycerolipid metabolism, PPAR signaling pathway