Heme oxygenase 2 (HMOX2) Mouse Monoclonal Antibody [Clone ID: OTI4H6]

CAT#: CF503824

Carrier-free (BSA/glycerol-free) HMOX2 mouse monoclonal antibody, clone OTI4H6 (formerly 4H6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HMOX2 (RC201777, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HMOX2. Positive lysates LY419512 (100ug) and LC419512 (20ug) can be purchased separately from OriGene.

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  HEK293T cells transfected with either RC201777 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HMOX2 antibody (TA503824), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI4H6
• Applications: FC, WB
• Recommended Dilution: WB 1:500, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human HMOX2(NP_002125) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 35.9 kDa
• Gene Name: heme oxygenase 2
• Database Link:
  NP_002125
  Entrez Gene 15369 MouseEntrez Gene 79239 RatEntrez Gene 3163 Human
  UniProt ID P30519
• Background: Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. Alternative splice variants encoding the same protein have been identified at this locus. [provided by RefSeq, Jul 2008]
• Synonyms: HO-2

Reference Data

• Protein Families: Transmembrane
• Protein Pathways: Porphyrin and chlorophyll metabolism