NAPE PLD (NAPEPLD) Mouse Monoclonal Antibody [Clone ID: OTI5F7]

CAT#: CF503860

Carrier-free (BSA/glycerol-free) NAPEPLD mouse monoclonal antibody, clone OTI5F7 (formerly 5F7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NAPEPLD (RC209877, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NAPEPLD. Positive lysates LY404703 (100ug) and LC404703 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NAPEPLD monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NAPEPLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503860)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NAPEPLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503860)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-NAPEPLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503860)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NAPEPLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503860)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-NAPEPLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503860)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-NAPEPLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503860)

• Anti-NAPEPLD mouse monoclonal antibody (TA503860) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NAPEPLD (RC209877).

• HEK293T cells transfected with either RC209877 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NAPEPLD antibody (TA503860), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI5F7
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NAPEPLD(NP_945341) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 45.4 kDa
• Gene Name: Homo sapiens N-acyl phosphatidylethanolamine phospholipase D (NAPEPLD), transcript variant 2, mRNA.
• Database Link:
  NP_945341
  Entrez Gene 242864 MouseEntrez Gene 296757 RatEntrez Gene 222236 Human
  UniProt ID Q6IQ20
• Background: NAPEPLD is a phospholipase D type enzyme that catalyzes the release of N-acylethanolamine (NAE) from N-acyl-phosphatidylethanolamine (NAPE) in the second step of the biosynthesis of N-acylethanolamine (Okamoto et al., 2004 [PubMed 14634025]). [supplied by OMIM, Oct 2008]
• Synonyms: FMP30; NAPE-PLD