CCK4 (PTK7) Mouse Monoclonal Antibody [Clone ID: OTI2E7]

CAT#: CF503920

Carrier-free (BSA/glycerol-free) PTK7 mouse monoclonal antibody, clone OTI2E7 (formerly 2E7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PTK7 (Cat# RC223416, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTK7(Cat# TA503920).

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PTK7 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Anti-PTK7 mouse monoclonal antibody (TA503920) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PTK7 (RC223416).

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  HEK293T cells transfected with either RC223416 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PTK7 antibody (TA503920), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI2E7
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:200~500, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PTK7(NP_690619) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: protein tyrosine kinase 7 (inactive)
• Database Link:
  NP_690619
  Entrez Gene 71461 MouseEntrez Gene 5754 Human
  UniProt ID Q13308
• Background: Receptor protein tyrosine kinases transduce extracellular signals across the cell membrane. A subgroup of these kinases lack detectable catalytic tyrosine kinase activity but retain roles in signal transduction. The protein encoded by this gene is a member of this subgroup of tyrosine kinases and may function as a cell adhesion molecule. This gene is thought to be expressed in colon carcinomas but not in normal colon, and therefore may be a marker for or may be involved in tumor progression. Four transcript variants encoding four different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: CCK-4; CCK4

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transmembrane