N Cadherin (CDH2) Mouse Monoclonal Antibody [Clone ID: OTI2G7]

CAT#: CF503933

Carrier-free (BSA/glycerol-free) CDH2 mouse monoclonal antibody, clone OTI2G7 (formerly 2G7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDH2 (RC207170, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDH2. Positive lysates LY419743 (100ug) and LC419743 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CDH2 monoclonal antibody.

• 

  Western blot analysis of extracts (35ug) from 6 cell lines by using anti-CDH2 monoclonal antibody (1:500).

• 

  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and CDH2-Knockout 293T cells (KO, Cat# LC840285) were separated by SDS-PAGE and immunoblotted with anti-CDH2 monoclonal antibody TA503933, (1:500). Then the blotted membrane was stripped and reprobed with anti-b-actin antibody (TA811000) as a loading control.

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-CDH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503933) (1:150)

• 

  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CDH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503933) (1:150)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CDH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503933) (1:150)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CDH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503933) (1:150)

• Living HEK293T cells transfected with either RC207170 plasmid (red) or empty vector (blue) were immunostained by anti-CDH2 antibody (TA503933) or isotype control antibody (green), and then analyzed by flow cytometry (1:50).

• Flow cytometric Analysis of Hela cells, using anti-CDH2 antibody (TA503933), (Red), compared to a nonspecific negative control antibody, (Blue).

Show all 10 images

Specifications

Product Data

• Clone Name: OTI2G7
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CDH2(NP_001783) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 97.2 kDa
• Gene Name: Homo sapiens cadherin 2 (CDH2), transcript variant 1, mRNA.
• Database Link:
  NP_001783
  Entrez Gene 12558 MouseEntrez Gene 83501 RatEntrez Gene 480169 DogEntrez Gene 711526 MonkeyEntrez Gene 1000 Human
  UniProt ID P19022
• Background: This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. [provided by RefSeq]
• Synonyms: CD325; CDHN; CDw325; NCAD

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Cell adhesion molecules (CAMs)