DPP9 Mouse Monoclonal Antibody [Clone ID: OTI2F10]

CAT#: CF503937

Carrier-free (BSA/glycerol-free) DPP9 mouse monoclonal antibody, clone OTI2F10 (formerly 2F10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DPP9 (RC224465, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DPP9. Positive lysates LY403380 (100ug) and LC403380 (20ug) can be purchased separately from OriGene.

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  Figure from citation: Western blot analysis of DPP9 protein expression by using anti-DPP9 antibody in Huh7 cells were transfected with DPP9 siRNA or nontargeting (NT) control siRNA for 48 h, or treated with enzyme inhibitor, 8 uM 1G244 (or vehicle control, 0.08% DMSO) for 6 h before incubating on fibronectin-coated plastic for 30 min. Dilution: 1:2000 View Citation

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  Anti-DPP9 mouse monoclonal antibody (TA503937) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DPP9 (RC224465).

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  HEK293T cells transfected with either RC224465 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DPP9 antibody (TA503937), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI2F10
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DPP9(NP_631898) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 96.4 kDa
• Gene Name: Homo sapiens dipeptidyl peptidase 9 (DPP9), transcript variant 1, mRNA.
• Database Link:
  NP_631898
  Entrez Gene 224897 MouseEntrez Gene 301130 RatEntrez Gene 91039 Human
  UniProt ID Q86TI2
• Background: This gene encodes a protein that is a member of the S9B family in clan SC of the serine proteases. The protein has been shown to have post-proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-termini of proteins. Although the activity of this protein is similar to that of dipeptidyl peptidase 4 (DPP4), it does not appear to be membrane bound. In general, dipeptidyl peptidases appear to be involved in the regulation of the activity of their substrates and have been linked to a variety of diseases including type 2 diabetes, obesity and cancer. Several transcript variants of this gene have been described but not fully characterized. [provided by RefSeq]
• Synonyms: DP9; DPLP9; DPRP-2; DPRP2

Reference Data

• Protein Families: Druggable Genome, Protease