SDS Mouse Monoclonal Antibody [Clone ID: OTI3D3]

CAT#: CF503943

Carrier-free (BSA/glycerol-free) SDS mouse monoclonal antibody, clone OTI3D3 (formerly 3D3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SDS (RC217814, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SDS. Positive lysates LY416388 (100ug) and LC416388 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-SDS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503943)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SDS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503943)

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  Anti-SDS mouse monoclonal antibody (TA503943) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SDS (RC217814).

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  HEK293T cells transfected with either RC217814 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SDS antibody (TA503943), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Jurkat cells, using anti-SDS antibody (TA503943), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI3D3
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SDS(NP_006834) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 34.4 kDa
• Gene Name: Homo sapiens serine dehydratase (SDS), mRNA.
• Database Link:
  NP_006834
  Entrez Gene 10993 Human
  UniProt ID P20132
• Background: This gene encodes one of three enzymes that are involved in metabolizing serine and glycine. L-serine dehydratase converts L-serine to pyruvate and ammonia and requires pyridoxal phosphate as a cofactor. The encoded protein can also metabolize threonine to NH4+ and 2-ketobutyrate. The encoded protein is found predominantly in the liver. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: SDH

Reference Data

• Protein Pathways: Cysteine and methionine metabolism, Glycine, serine and threonine metabolism, Metabolic pathways