PDSS2 Mouse Monoclonal Antibody [Clone ID: OTI1C11]

CAT#: CF503976

Carrier-free (BSA/glycerol-free) PDSS2 mouse monoclonal antibody, clone OTI1C11 (formerly 1C11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDSS2 (RC207892, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDSS2. Positive lysates LY412512 (100ug) and LC412512 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PDSS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503976)

• 

  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PDSS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503976)

• 

  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PDSS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503976)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PDSS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503976)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PDSS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503976)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PDSS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503976)

Show all 7 images

Specifications

Product Data

• Clone Name: OTI1C11
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PDSS2(NP_065114) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43.9 kDa
• Gene Name: Homo sapiens decaprenyl diphosphate synthase subunit 2 (PDSS2), mRNA.
• Database Link:
  NP_065114
  Entrez Gene 71365 MouseEntrez Gene 365592 RatEntrez Gene 57107 Human
  UniProt ID Q86YH6
• Background: The protein encoded by this gene is an enzyme that synthesizes the prenyl side-chain of coenzyme Q, or ubiquinone, one of the key elements in the respiratory chain. The gene product catalyzes the formation of all trans-polyprenyl pyrophosphates from isopentyl diphosphate in the assembly of polyisoprenoid side chains, the first step in coenzyme Q biosynthesis. Defects in this gene are a cause of coenzyme Q10 deficiency. COMPLETENESS: complete on the 3' end.
• Synonyms: bA59I9.3; C6orf210; COQ10D3; DLP1; hDLP1

Reference Data

• Protein Pathways: Terpenoid backbone biosynthesis