HES1 Mouse Monoclonal Antibody [Clone ID: OTI2D2]

CAT#: CF504001

Carrier-free (BSA/glycerol-free) HES1 mouse monoclonal antibody, clone OTI2D2 (formerly 2D2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HES1 (RC211709, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HES1 (1:500).

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-HES1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504001)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-HES1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504001)

• 

  Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-HES1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504001)

• 

  Anti-HES1 mouse monoclonal antibody (TA504001) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HES1 (RC211709).

• 

  Flow cytometric Analysis of Jurkat cells, using anti-HES1 antibody (TA504001), (Red), compared to a nonspecific negative control antibody, (Blue).

• Western blot analysis of extracts (10ug) from 8 different cell lines by using anti-HES1 monoclonal antibody (1:200).

• Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-HES1 monoclonal antibody (1:200).

Show all 8 images

Specifications

Product Data

• Clone Name: OTI2D2
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinnat protein of human HES1(NP_005515) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29.4 kDa
• Gene Name: Homo sapiens hes family bHLH transcription factor 1 (HES1), mRNA.
• Database Link:
  NP_005515
  Entrez Gene 15205 MouseEntrez Gene 29577 RatEntrez Gene 3280 Human
  UniProt ID Q14469
• Background: This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box. [provided by RefSeq]
• Synonyms: bHLHb39; HES-1; HHL; HRY

Reference Data

• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway, Transcription Factors
• Protein Pathways: Maturity onset diabetes of the young, Notch signaling pathway