FBXO31 Mouse Monoclonal Antibody [Clone ID: OTI1A11]

CAT#: CF504012

Carrier-free (BSA/glycerol-free) FBXO31 mouse monoclonal antibody, clone OTI1A11 (formerly 1A11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FBXO31 (RC203518, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FBXO31. Positive lysates LY411131 (100ug) and LC411131 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FBXO31 monoclonal antibody.

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  HEK293T cells transfected with either RC203518 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FBXO31 antibody (TA504012), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1A11
• Applications: FC, WB
• Recommended Dilution: WB 1:500~2000, FLOW 1:100
• Reactivities: Human, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human FBXO31(NP_079011) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 60.5 kDa
• Gene Name: Homo sapiens F-box protein 31 (FBXO31), transcript variant 1, mRNA.
• Database Link:
  NP_079011
  Entrez Gene 76454 MouseEntrez Gene 498959 RatEntrez Gene 479620 DogEntrez Gene 79791 Human
  UniProt ID Q5XUX0
• Background: Members of the F-box protein family, such as FBXO31, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]). [supplied by OMIM]
• Synonyms: FBX14; Fbx31; FBXO14; MRT45; pp2386

Reference Data

• Protein Families: Druggable Genome