TPSG1 Mouse Monoclonal Antibody [Clone ID: OTI1E6]

CAT#: CF504027

Carrier-free (BSA/glycerol-free) TPSG1 mouse monoclonal antibody, clone OTI1E6 (formerly 1E6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TPSG1 (RC222359, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TPSG1. Positive lysates LY402219 (100ug) and LC402219 (20ug) can be purchased separately from OriGene.

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  HEK293T cells transfected with either RC222359 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TPSG1 antibody (TA504027), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1E6
• Applications: FC, WB
• Recommended Dilution: WB 1:2000, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 20-283 of human TPSG1(NP_036599) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 32 kDa
• Gene Name: Homo sapiens tryptase gamma 1 (TPSG1), mRNA.
• Database Link:
  NP_036599
  Entrez Gene 25823 Human
  UniProt ID Q9NRR2
• Background: Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. There is uncertainty regarding the number of genes in this cluster. Currently four functional genes - alpha I, beta I, beta II and gamma I - have been identified. And beta I has an allelic variant named alpha II, beta II has an allelic variant beta III, also gamma I has an allelic variant gamma II. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha-tryptases predominant. This gene differs from other members of the tryptase gene family in that it has C-terminal hydrophobic domain, which may serve as a membrane anchor. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: PRSS31; TMT; trpA

Reference Data

• Protein Families: Druggable Genome, Transmembrane