SIX1 Mouse Monoclonal Antibody [Clone ID: OTI1H4]

CAT#: CF504057

Carrier-free (BSA/glycerol-free) SIX1 mouse monoclonal antibody, clone OTI1H4 (formerly 1H4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIX1 (RC203465, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIX1. Positive lysates LY401814 (100ug) and LC401814 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-SIX1 monoclonal antibody at 1:200 dilution.

Specifications

Product Data

• Clone Name: OTI1H4
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-284 of human SIX1(NP_005973) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 32 kDa
• Gene Name: Homo sapiens SIX homeobox 1 (SIX1), mRNA.
• Database Link:
  NP_005973
  Entrez Gene 20471 MouseEntrez Gene 114634 RatEntrez Gene 6495 Human
  UniProt ID Q15475
• Background: The protein encoded by this gene is a homeobox protein that is similar to the Drosophila 'sine oculis' gene product. This gene is found in a cluster of related genes on chromosome 14 and is thought to be involved in limb development. Defects in this gene are a cause of autosomal dominant deafness type 23 (DFNA23) and branchiootic syndrome type 3 (BOS3). [provided by RefSeq]
• Synonyms: BOS3; DFNA23; TIP39

Reference Data

• Protein Families: Transcription Factors