CCK4 (PTK7) Mouse Monoclonal Antibody [Clone ID: OTI2H4]

CAT#: CF504076

Carrier-free (BSA/glycerol-free) PTK7 mouse monoclonal antibody, clone OTI2H4 (formerly 2H4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTK7 (RC223416, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTK7.

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  Anti-PTK7 mouse monoclonal antibody (TA504076) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PTK7 (RC223416).

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  HEK293T cells transfected with either RC223416 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PTK7 antibody (TA504076), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI2H4
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PTK7(NP_690619) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens protein tyrosine kinase 7 (inactive) (PTK7), transcript variant PTK7-2, mRNA.
• Database Link:
  NP_690619
  Entrez Gene 71461 MouseEntrez Gene 5754 Human
  UniProt ID Q13308
• Background: This gene encodes a member of the receptor protein tyrosine kinase family of proteins that transduce extracellular signals across the cell membrane. The encoded protein lacks detectable catalytic tyrosine kinase activity, is involved in the Wnt signaling pathway and plays a role in multiple cellular processes including polarity and adhesion. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]
• Synonyms: CCK-4; CCK4

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transmembrane